Thymosin Beta-4 Fragment

TB-500 Dosage Calculator & Guide

A recovery-focused peptide used in healing protocols. Calculate your dose and see the difference between loading and maintenance phases.

Updated April 2026·5 min read·For educational purposes only

TB-500 at a Glance

Also Known As
Thymosin Beta-4, TB4, Timbetasin
Developer
Research peptide (naturally occurring)
Mechanism
Actin Regulation / Cell Migration
Injection Frequency
2-3x Weekly (loading), 1-2x (maintenance)
Common Vial Sizes
2 mg, 5 mg, 10 mg
Storage (Reconstituted)
Refrigerate, 4-6 weeks
Route
Subcutaneous / Intramuscular
Half-Life
~24 hours

TB-500 Dosage Calculator

Pre-filled with typical tb-500 values. Adjust to match your vial and protocol.

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What Is TB-500?

TB-500 is a synthetic version of Thymosin Beta-4 (TB4), a naturally occurring 43-amino-acid peptide found in virtually all human and animal cells. Thymosin Beta-4 plays a critical role in cell differentiation, migration, and tissue repair throughout the body.

TB-500 specifically reproduces the active region of TB4 responsible for actin binding, cell migration, blood vessel formation, and reduction of inflammation. It has been studied extensively in veterinary medicine (particularly in racehorses) and has gained popularity in human performance and recovery applications for its ability to promote systemic healing of muscles, tendons, ligaments, skin, and even cardiac tissue.

Important: TB-500 has not been approved by the FDA for general consumer use. The information on this page is for educational and research purposes only. Always consult a qualified healthcare provider before using any peptide.

TB-500 Dosing Protocol

Below are common dosing protocols for tb-500 based on available research and community experience:

ProtocolDoseNotes
Loading Phase (Weeks 1-4)5-10 mg/weekSplit into 2-3 injections; accelerates initial healing
Maintenance Phase2-5 mg/week1-2 injections per week
Injury Protocol2.5 mg 2x/weekCommon moderate dosing for active injuries
Typical Duration6-12 weeksLoading 4-6 weeks, then maintenance
Tip: Use the calculator above to recalculate your syringe units whenever you adjust your dose. Bookmark this page for quick reference.

Ready to calculate? Use the calculator above or our full calculator with syringe visualization.

How to Reconstitute TB-500

TB-500 comes as a lyophilized (freeze-dried) powder that must be reconstituted with bacteriostatic water before use. The process is standard for all peptides:

For a detailed step-by-step guide with visuals, see our complete peptide reconstitution guide.

TB-500 Side Effects

Understanding potential side effects helps you use tb-500 more safely. Most side effects are dose-dependent and may improve over time.

Common Side Effects

Less Common Side Effects

If you experience severe or persistent side effects, discontinue use and contact your healthcare provider.

How to Store TB-500

Before reconstitution: Store lyophilized (powder) tb-500 in a cool, dry place away from direct sunlight. Refrigeration is ideal for long-term storage of unreconstituted peptides.

After reconstitution: Store the reconstituted vial upright in a refrigerator at 36-46°F (2-8°C). Do not freeze. The solution typically remains stable for 4-6 weeks. Always swab the vial stopper with an alcohol pad before each use.

Loading vs. Maintenance Protocol

TB-500 protocols typically follow a two-phase approach. The loading phase (weeks 1-4 or 1-6) uses higher doses (5-10 mg per week, split across 2-3 injections) to rapidly build systemic levels. The maintenance phase then drops to a lower dose (2-5 mg per week, 1-2 injections) to sustain healing.

For example, a common 8-week protocol might look like: Weeks 1-4: 2.5 mg injected Monday and Thursday (5 mg/week total). Weeks 5-8: 2.5 mg injected once per week. Use the calculator above and adjust the dose field for each phase.

TB-500 FAQ

What's the difference between TB-500 and Thymosin Beta-4?+
TB-500 is a synthetic fragment that reproduces the active healing region of the full-length Thymosin Beta-4 protein. While they share the same core mechanism, TB-500 specifically targets the actin-binding domain responsible for tissue repair. In practice, the terms are often used interchangeably.
Does it matter where I inject TB-500?+
Unlike BPC-157, TB-500 is considered systemically active regardless of injection site. This means subcutaneous injection in the abdomen, thigh, or arm should all produce similar effects. TB-500 migrates to areas of injury and inflammation throughout the body.
Should I combine TB-500 with BPC-157?+
The BPC-157 + TB-500 combination is one of the most popular healing stacks. They work through complementary pathways, with TB-500 used for broader recovery and BPC-157 often used for more local tissue support. See our BPC-157 + TB-500 stack guide or the BPC-157 calculator.
What does the 'loading phase' mean?+
The loading phase involves taking a higher dose for the first 4-6 weeks to build up tissue levels of TB-500. Because TB-500 has a relatively short half-life, a loading phase helps establish therapeutic concentrations faster. After loading, the maintenance phase uses lower doses to sustain those levels.
Is TB-500 legal?+
TB-500 is legal to purchase for research purposes in most countries. It is not FDA-approved for human use. It is banned by WADA (World Anti-Doping Agency) and most sports organizations. Always check the rules of your sport or governing body before use.

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